FABRICATION AND CHARACTERIZATION OF PLASTIC MICROFLUID DEVICES MODIFIED WITH POLYELECTROLYTE MULTILAYERS

摘要

Microchannels fabricated in plastic materials by room temperature imprinting demonstrate large variability in surface charge as a result of the fabrication procedure. We have determined by chemical mapping that surface charged groups are primarily localized on the channel side walls and not on the channel floor. Due to this variation in charge across the imprinted plastic microchannel, there is differential adsorption of protein that is dependent on the location in the channel. Polyelectrolyte multilayers (PEMs) can be used to effectively coat the plastic microchannel surface with high reproduciblity and excellent stability. We show that polyelectrolyte multilayers can be used to alter both direction and rate of flow in plastic microchannel devices. This paper addresses the fabrication of plastic microfluid channels, chemical characterization of channel charge, determination of electroosmotic flow, and the effect of channel derivatization with PEMs on flow and protein immobilization.