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Biosensors for Detecting Stress in Developing Embryos

机译:用于检测胚胎发育中压力的生物传感器

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摘要

Reactive Oxygen Species (ROS) cause DNA damage and defective function in sperm and also affects the developmental competence of embryos. It is therefore critical to monitor ROS in sperm, oocytes and developing embryos. In particular, hydrogen peroxide (H_2O_2) is a ROS important to normal cell function and signalling as well as its role in oxidative stress. Here we report the development of a fluorescent sensor for H_2O_2 using carboxyperoxyfluor-1 (CPF1) in solution and attached to a glass slide or multi-mode optical fibre. CPF1 increases in fluorescence upon reaction with H2O2 to non-invasively detect H2O2 near developing embryos. These probes are constructed by immobilising CPF1 to the optical fibre tip a polyacrylamide layer. Also reported is a new dual optical fibre sensor for detecting both H_2O_2 and pH that is functional at biologically concentrations of H_2O_2 and can sense pH to 0.1 units. This research shows promise for the use of optical fibre sensors for monitoring the health of developing embryos. Furthermore, these sensors are applicable for use beyond embryos such as detecting stress in endothelial cells involved in cardiovascular dysfunction.
机译:活性氧(ROS)导致精子中的DNA损伤和功能缺陷,还影响胚胎的发育能力。因此,监测精子,卵母细胞和发育中的胚胎中的ROS至关重要。特别是过氧化氢(H_2O_2)是对正常细胞功能和信号转导及其在氧化应激中的作用重要的ROS。在这里,我们报告了在溶液中使用羧基过氧氟-1(CPF1)并将其连接到载玻片或多模光纤上的H_2O_2荧光传感器的开发。与H2O2反应后,CPF1的荧光增加,从而可以无创地检测发育中的胚胎附近的H2O2。这些探针是通过将CPF1固定在光纤尖端的聚丙烯酰胺层构成的。还报道了一种用于检测H_2O_2和pH的新型双光纤传感器,该传感器在H_2O_2的生物浓度下具有功能,可以感应到0.1个单位的pH。这项研究表明有望将光纤传感器用于监测发育中的胚胎的健康状况。此外,这些传感器适用于胚胎以外的用途,例如检测参与心血管功能障碍的内皮细胞中的压力。

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  • 来源
    《SPIE biophotonics Australasia》|2016年|100130b.1-100130b.6|共6页
  • 会议地点 Adelaide(AU)
  • 作者单位

    ARC Centre of Excellence in Nanoscale BioPhotonics (CNBP),Institute for Photonics and Advanced Sensing (IPAS), The University of Adelaide, North Terrace, Adelaide 5005, Australia,Heart Health Theme, South Australian Health and Medical Research Institute (SAHMRI), North Terrace, Adelaide 5001, Australia;

    ARC Centre of Excellence in Nanoscale BioPhotonics (CNBP),Institute for Photonics and Advanced Sensing (IPAS), The University of Adelaide, North Terrace, Adelaide 5005, Australia,The Robinson Institute, School of Paediatrics and Reproductive Health, The University of Adelaide, North Terrace, Adelaide 5005, Australia;

    ARC Centre of Excellence in Nanoscale BioPhotonics (CNBP),Institute for Photonics and Advanced Sensing (IPAS), The University of Adelaide, North Terrace, Adelaide 5005, Australia,The Robinson Institute, School of Paediatrics and Reproductive Health, The University of Adelaide, North Terrace, Adelaide 5005, Australia;

    ARC Centre of Excellence in Nanoscale BioPhotonics (CNBP),Heart Health Theme, South Australian Health and Medical Research Institute (SAHMRI), North Terrace, Adelaide 5001, Australia;

    ARC Centre of Excellence in Nanoscale BioPhotonics (CNBP),Institute for Photonics and Advanced Sensing (IPAS), The University of Adelaide, North Terrace, Adelaide 5005, Australia;

    ARC Centre of Excellence in Nanoscale BioPhotonics (CNBP),Institute for Photonics and Advanced Sensing (IPAS), The University of Adelaide, North Terrace, Adelaide 5005, Australia,The Robinson Institute, School of Paediatrics and Reproductive Health, The University of Adelaide, North Terrace, Adelaide 5005, Australia;

    ARC Centre of Excellence in Nanoscale BioPhotonics (CNBP),Institute for Photonics and Advanced Sensing (IPAS), The University of Adelaide, North Terrace, Adelaide 5005, Australia,The Robinson Institute, School of Paediatrics and Reproductive Health, The University of Adelaide, North Terrace, Adelaide 5005, Australia;

    ARC Centre of Excellence in Nanoscale BioPhotonics (CNBP),University of South Australia, North Terrace, Adelaide 5001, Australia;

    ARC Centre of Excellence in Nanoscale BioPhotonics (CNBP),Heart Health Theme, South Australian Health and Medical Research Institute (SAHMRI), North Terrace, Adelaide 5001, Australia;

    ARC Centre of Excellence in Nanoscale BioPhotonics (CNBP),Institute for Photonics and Advanced Sensing (IPAS), The University of Adelaide, North Terrace, Adelaide 5005, Australia;

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  • 入库时间 2022-08-26 13:45:26

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