首页> 外文会议>Spectral Imaging: Instrumentation, Applications, and Analysis III; Progress in Biomedical Optics and Imaging; vol.6, no.9 >Instrumentation considerations in spectral imaging for tissue demarcation: comparing three methods of spectral resolution
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Instrumentation considerations in spectral imaging for tissue demarcation: comparing three methods of spectral resolution

机译:光谱成像中用于组织划界的仪器注意事项:比较三种光谱分辨率方法

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Multiple methodologies exist to implement spectral imaging for tissue demarcation and disease diagnosis. In this paper, benchtop acousto-optic tunable filter (AOTF), liquid-crystal tunable filter (LCTF) and Fourier interferometric spectral imaging systems were quantitatively compared in terms of imaging speed of soft tissue autofluorescence. Optical throughput, image signal-to-noise ratio (SNR), and collagen autofluorescence imaging in chicken breast were assessed. Within this comparison, the Fourier system possessed the largest optical throughput (~50%) relative to the tunable-filter imaging systems; however, its throughput advantage failed to correlate to improved image SNR over the LCTF system. Further, while the autofluorescence imaging capability of the Fourier system exceeded that of the LCTF system for comparable total image integration times, the LCTF is capable of producing equivalent autofluorescence SNR with superior SNR when interrogations at only a few wavelengths are required and the random access filter tuning of the LCTF can be exploited. Therefore, the simple, rugged design and random-access filter-tuning capability of LCTF-based spectral imaging makes it best-suited for clinical development of soft tissue autofluorescence imaging.
机译:存在多种方法来实现用于组织划分和疾病诊断的光谱成像。本文从软组织自发荧光的成像速度上定量比较了台式声光可调滤光片(AOTF),液晶可调滤光片(LCTF)和傅立叶干涉光谱成像系统。评估了鸡胸肉的光通量,图像信噪比(SNR)和胶原自发荧光成像。在此比较中,相对于可调滤波器成像系统,傅里叶系统具有最大的光通量(〜50%);但是,其吞吐量优势未能与LCTF系统上改善的图像SNR相关。此外,尽管在可比较的总图像积分时间上,傅里叶系统的自发荧光成像能力超过了LCTF系统,但当仅需要几个波长的询问和随机访问滤波器时,LCTF能够产生等效的自发荧光SNR,且具有出色的SNR。可以利用LCTF的调整。因此,基于LCTF的光谱成像的简单,坚固的设计和随机存取滤波器的调谐功能使其最适合于软组织自发荧光成像的临床开发。

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