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Multispectral Endoscopy and Microscopy Imaging Using a Spectrally Programmable Light Engine

机译:使用光谱可编程光引擎进行多光谱内窥镜和显微镜成像

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摘要

We report a spectrally and temporally programmable light engine based on a spatial light modulator that can dynamically create any narrow or broadband spectral profile for hyperspectral, fluorescence, or principal component imaging. Most hyperspectral or multispectral imaging systems use wavelength selection devices such as acousto-optic tunable filters (AOTFs), tunable grating or prism-based monochromators, or filter wheels. While these devices can select wavelengths they cannot create arbitrary spectral profiles. This simple and economical system can be controlled at high speed (up to 5000 illumination profiles per second). Digitally controlled illumination is bit additive with image data providing high dynamic range imaging with monochrome or color imaging devices. This is especially advantageous for endoscopes employing small well CCD or CMOS sensors since the dynamic range now can extend beyond the limits of the sensor itself. In this report we show multispectral images of in vivo tissue and in vitro tissue samples using endoscopes, surgical microscopes and conventional microscopes.
机译:我们报告了一种基于空间光调制器的光谱和时间可编程光引擎,该光调制器可以动态创建用于高光谱,荧光或主成分成像的任何窄或宽带光谱图。大多数高光谱或多光谱成像系统使用波长选择设备,例如声光可调滤光片(AOTF),可调光栅或基于棱镜的单色仪或滤光轮。虽然这些设备可以选择波长,但它们不能创建任意光谱轮廓。这种简单而经济的系统可以进行高速控制(每秒高达5000个照明配置文件)。数控照明是图像数据的位加法运算,可通过单色或彩色成像设备提供高动态范围成像。这对于采用小尺寸CCD或CMOS传感器的内窥镜特别有利,因为动态范围现在可以扩展到传感器本身的范围之外。在此报告中,我们使用内窥镜,手术显微镜和常规显微镜显示了体内组织和体外组织样品的多光谱图像。

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