Integrated Elastomer Fluidic Lab-on-a-chip - Surface Patterning and DNA Diagnostics

摘要

We recently developed a method of multilayer fabrication forrnelastomeric devices, which we used to fabricate monolithic activernvalves and pumps. Here we describe efforts to use these pumpsrnand valves in an integrated DNA diagnostic chip and show resultsrnof a key component, surface patterning, with two different kind ofrnsurface chemistries by using similar elastomeric channel devices.rnFlow control, reagent metering, in-line mixing and looprncirculations are also demonstrated.