The Quantum Dot nanoconjugate toolbox

摘要

The surface coating of quantum dots has been characterised using Z-stem. Quantum dots have been pegylated toincrease stability in aqueous solution. The fluorescence intensity of the quantum dots was modulated pegylation. PEGwas coupled using different ratios of EDC, PEG and NHS. Optimum coupling conditions were found to occur when2000 equivalents of PEG were reacted with 1 equivalent of dot in the presence of 1500 equivalents of NHS and EDC.Angiotensin II was also conjugated to quantum dots and these conjugates were shown to be biologically active.Quantum dots have also been surface functionalised with other peptides such as NGR with subsequent demonstration ofcell surface binding and can be characterized by flow cytometry.