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Chemiluminescence and fluorescence spectrum methods for determination of Aflatoxin B1 mediated by “FCLA + BSA”

机译:化学发光和荧光光谱法测定“ FCLA + BSA”介导的黄曲霉毒素B1

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BSA (Bovine Serum Albumin) can enlarge the CL intensity of FCLA(3,7-dihydro-6-{4-{2-(N’–(5-fluoresceinyl)thioureido)ethoxy}phenyl}-2-methylimi-dazo{1,2-a}pyrazin-3-one dosium salt) to 763%. This report presents novelmethods for determination of Aflatoxin B1 (AfB1) mediated by FCLA+BSA. The concentration of AFB1 showed anobvious positive correlation with the chemiluminescence (CL) intensity mediated by FCLA+BSA, correlativecoefficient R≅0.94. This method could measure accurately ng/ml of AfB1 concentration. 365nm as excitatedwavelength, 440nm and 520nm-two fluorescence peaks of FCLA+BSA+AfB1 were found. The fluorescence intensityof peak at 440nm showed an obvious positive correlation with the concentration of AFB1, R≅0.97; the fluorescenceintensity of peak at 520nm showed a positive correlation with the concentration of AFB1, R≅0.90. Comparing the peakof FCLA, FCLA+BSA and FCLA+BSA+AfB1 had a 6nm Einstein shift (red shift). The study suggested that CL andfluorescence spectrum methods mediated by FCLA+BSA might be applicable to the determination of AfB1concentration.
机译:牛血清白蛋白(BSA)可以增强FCLA(3,7-二氢-6- {4- {2-(N'-(5-'5-氟乙烯基)硫脲基)乙氧基}苯基}苯基} -2-甲基咪唑{ 1,2-a}吡嗪-3-一one盐)至763%。本报告介绍了测定由FCLA + BSA介导的黄曲霉毒素B1(AfB1)的新方法。 AFB1的浓度与FCLA + BSA介导的化学发光(CL)强度呈明显的正相关,相关系数R.0.94。该方法可以准确测量ng / ml的AfB1浓度。激发波长为365nm,发现了440nm和520nm两个FCLA + BSA + AfB1的荧光峰。 440nm处的峰值荧光强度与AFB1的浓度呈显着正相关,R≅0.97; 520nm处荧光峰强度与AFB1浓度呈正相关,R≅0.90。比较FCLA,FCLA + BSA和FCLA + BSA + AfB1的峰有6nm爱因斯坦位移(红移)。研究表明,FCLA + BSA介导的CL和荧光光谱方法可能适用于AfB1浓度的测定。

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