首页> 外文会议>Smart Medical and Biomedical Sensor Technology III >Development of an electrochemical biosensor without a sandwich assay
【24h】

Development of an electrochemical biosensor without a sandwich assay

机译:无需夹心分析的电化学生物传感器的开发

获取原文
获取原文并翻译 | 示例

摘要

The combination of electrochemistry with microfluidic sample processing is a viable option to reduce the size, logistics load and power consumption of biosensors. Modern microfluidics technology makes it possible to perform sample clean-up, PCR, sample concentration and transduction on the same disposable chip. This presentation will discuss two novel electrochemical techniques which do not require a sandwich assay and can be employed on a disposable microfluidic chip, reducing logistics load and microfluidic complexity. Transduction is achieved via an electrochemical DNA hybridization sensor similar to a molecular beacon removing the need for a sandwich assay also referred to as E-DNA. The sensor is designed where a DNA stem-loop structure is immobilized on a gold electrode with a redox label held close to the surface. Upon hybridization the stem-loop opens and the label pulls away from the surface so that current cannot flow to the electrode under positive bias. This paper will primarily discuss experiments trying to understand the hybridization event and effect of surface morphology on electrochemical signal transduction.
机译:电化学与微流体样品处理相结合是减少生物传感器尺寸,减少物流负荷和降低功耗的可行选择。现代微流体技术使在同一块一次性芯片上进行样品净化,PCR,样品浓缩和转导成为可能。本演讲将讨论两种不需要夹心测定的新型电化学技术,这些技术可用于一次性微流控芯片上,从而减少物流负荷和微流控复杂性。通过类似于分子信标的电化学DNA杂交传感器实现转导,无需进行夹心测定(也称为E-DNA)。该传感器的设计是将DNA茎环结构固定在金电极上,并在表面上保持氧化还原标记。杂交后,茎环打开,标签从表面拉开,因此电流不能在正偏压下流到电极。本文将主要讨论试图了解杂交事件和表面形态对电化学信号转导的影响的实验。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号