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Functional Attachment of Horse Radish Peroxidase to Plasma Treated Surfaces

机译:辣根过氧化物酶对血浆处理表面的功能附着

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Controlling the interaction of surfaces with macromolecules, such as proteins and antibodies, is the key to producing biocompatible prosthetic devices, biosensors and diagnostic arrays. The development of technologies to control these interactions will result in the early detection of disease and have the potential to dramatically reduce costs associated with clinical treatment. For example, tethering functional anti-bodies to a surface in a patterned array allows the selection of specific proteins from a microlitre serum sample, immediately identifying diseases, well before the symptoms are manifested. Unfortunately, simple physical absorption of proteins onto most surfaces results in changes in their structure and loss of function. The use of ions from plasmas allows flexibility in surface modification by accessing a variety of ion energies and activated chemical species. In this paper we describe plasma based techniques which are being developed to modify the chemistry and morphology of surfaces in order to optimise their interaction with biomolecules. Early results of plasma processes to activate surfaces for non specific attachment of proteins by hydrophilic /hydrophobic interactions are presented, with particular attention to the time stability of such treatments, which is of special interest.
机译:控制表面与大分子(例如蛋白质和抗体)的相互作用是生产生物相容性修复设备,生物传感器和诊断阵列的关键。控制这些相互作用的技术的发展将导致疾病的早期发现,并具有显着降低与临床治疗相关的成本的潜力。例如,将功能性抗体束缚在图案化阵列的表面上可以从微升血清样品中选择特定蛋白质,并在症状显现之前立即识别疾病。不幸的是,蛋白质在大多数表面上的简单物理吸收导致其结构改变和功能丧失。来自等离子体的离子的使用通过获取各种离子能量和活化的化学物质而在表面改性方面具有灵活性。在本文中,我们描述了基于等离子体的技术,这些技术正在开发中,旨在改变表面的化学和形态,以优化它们与生物分子的相互作用。提出了通过亲水/疏水相互作用激活蛋白质非特异性附着表面的等离子体处理的早期结果,特别注意这种处理的时间稳定性,这是特别令人关注的。

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