Genetic Diversity of Testa Pigments and RAPD Marker of Yellow-seeded Rapeseed (Brassica napus L.)

摘要

14 yellow-seeded rapeseed lines (Brassia napus L.) from different genetic sources were used to analyze diversity of testa pigments content, oil and protein content, and RAPD markers. The results showed: the anthocyanin and melanin were the most important pigments in testa and their content were responsible for the variation in seed color ranging from orange to black yellow, 14 yellow-seeded lines could be classified into 3 groups: high anthocyanin content group with anthocyanin content over 2.54 mg/g, the seed color were light yellow or orange; low pigments content group with low content of anthocyanin and melanin, the testa were transparent and the seed color were light yellow, greenish yellow or twany; high melanin content group with melanin content over 178.4U, the testa were black, the seed color is black yellow. Oil content changed from 36.2% to 45.5%, protein content from 21.1% to 27.7%, and the correlation analysis revealed the oil content is highly significantly negatively correlated with the protein content. The cluster analysis showed that the extensive genetic variation existed among 14 yellow-seeded lines by using unweighted paired group method with arithmetic average (UPGMA) based on 97 RAPD markers which were amplified with 21 decamer primers, the genetic similarity among them ranged from 0.25 to 0.909, and 14 yellow-seeded lines could put into 2 clusters corresponding to genome difference.