MIR-192 AND MIR-215 INHIBIT NUCLEOTIDE EXCISION REPAIR BY TARGETING ERCC3 IN A549 CELL LINE

摘要

Ionizing radiation(IR) plays an important role in the comprehensive therapy of non-small cell lung cancer, it can interfere the homeostasis of intracellular environment by extremely complex mechanisms, one of the most important one is to change the level of gene expression. MicroRNAs, which act at the posttranscriptional level via an RNA interference mechanism, is involved in the process of radiationinduced damage and repair, but the definite regulatory mechanism of microRNAs is still not clear. In this study, we investigated the expression level of miR-192,miR-215 and the protein ERCC3 in human lung carcinoma cell line A549 after radiation with 20Gy and 40Gy. Data analysis revealed that miR-192, miR-215 and ERCC3 protein consistently responded to ionizing radiation(IR),miR-192 and miR-215 were upregulated, and ERCC3 protein was down-regulated. We concluded that radiation ray may down regulate the expression of ERCC3 through up regulate the expression of miR-192 and miR-215,and then regulate the process of DNA damage and repair.