Controllable Preparation of 10 nm Solid Lipid Nanoparticles Based on Phase Behaviors of Drug-Loading Hot Microemulsions

摘要

A new method was presented here for controllable preparation of 10-nm-scale solid lipid nanoparticles (SLNs). The pseudoternary phase diagram for the system polyoxyethylene(40)stearate(S-40)/polyoxyethylene-b-poly oxypropylene(F-68)/ Glycerol monostearate(GMS)/ water was obtained at 60℃ using home-made apparatus with temperature control. Different microemulsions (W/O, B.C. and O/W) were distinguished by the conductivity measurement, and liquid crystal (LC) region was also found. The introduction of retinoic acid (RA) and temperature had little influence on the phase behaviors. RA-SLN was prepared by direct cooling of hot O/W microemulsion obtained according to the phase behavior results. The mean particle size of RA-SLN investigated by PCS was about 10 nm. TEM images indicated that RA-SLNs were spherical nanoparticles with diameter about 10 nm. It has been proved that the stability and the skin penetrating capacity of RA-SLN were very good.