Biomimetic Tissue Platform for Photothermal Cancer Therapy using Gold Nanorods (GNRs)

摘要

Photothermal therapy (PT) provides a strong potential in treatment of tumors, selective cell death, through the ability of gold nanoparticles to target destructive heat preferentially to tumor regions. And yet, clinical application of the thermal therapies has not accomplished due to insufficient processes of the heating methods and temperature measuring techniques leading to low reproducibility of such treatment. In this study, we created a 3 dimensional tissue platform to characterize the heating method and to control the generated heat in the tissue used for a superficial cancer model using gold nanorods (GNRs) and near-infrared (NIR, 808 nm) laser. The 3D tissue platform involved a 2 mm wide hemisphere to confine the GNRs covered with20 urn thick polymer film designed to mimic localized nanoparticles in tumor. Moreover, this platform provides an easy way to measure heat distribution and temperature created in tumor cross section. To investigate the photothermal effect of GNRs on heat generation, the amount of GNRs and laser power density were controlled. The GNRs were shown to be the large absorption cross sections generating localized photothermal effects and hyperthermic effects on destructive consequences in the cell dynamics causing a partial tumor regression.