SNP ANALYSIS BY DYNAMIC ALLELE SPECIFIC HYBRIDIZATION ON PATTERNED MONOLAYERS OF BEADS

摘要

In this study, the possibility of miniaturizing the novel SNP scoring method dynamic allele specific hybridization (DASH) onto immobilized monolayers of patterned beads is shown. Microcontact printing using elastomere poly(dimethylsiloxane) (PDMS) stamp was employed to create patterned monolayers of beads on a silicondioxide surface. DASH procedure, involving heating of DNA duplex, was performed and a denaturation profile of the DNA duplex was achieved. The bead-based DASH procedure presented here enables dramatic reduction of the working volumes and is a step towards developing cost-effective high-throughput DASH method on arrays of single beads.