Two-photon imaging and nanoprocessing of stem cells with sub-20 fs laser pulses

摘要

Novel ultracompact multiphoton sub-20 femtosecond near infrared MHz laser scanning microscopes and conveational 250 fs laser microscopes have been used to perform high resolution multi-photon imaging of stem cell clusters as well as targeted intracellular nanoprocessing and knock-out of living single stem cells within a 3D microenvironment. Also lethal exposure of large parts of cell clusters was successfully probed while maintaining single cells of interest alive. Mean powers in the milliwatt range for 3D nanoprocessing and microwatt powers for two-photon imaging were found to be sufficient. Ultracompact low power sub-20 fs laser systems may become interesting tools for nanobiotechnology such as optical cleaning of stem cell clusters and optical transfection.