Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser

摘要

A multimodal multiphoton microscopy (MPM) is developed to acquire two-photon and three-photon signalssimultaneously, including two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and thirdharmonic generation (THG). We have developed a miniature multimodal MPM system based on a dual-wavelength Erdopedfiber laser, which includes the fundamental pulse at 1580 nm to excite THG and the frequency-doubled pulse at790 nm to excite TPEF and SHG. The laser is coupled by a single mode fiber into a miniature MPM imaging probe.Label-free imaging by TPEF, SHG, and THG are demonstrated on biological samples, obtained from intrinsicfluorophores, collagen, lipid and interface.