Fast Fourier transform second harmonic generation microscopy for probing the 3D structure of corneal lamella

摘要

Cornea is one of the collagen-rich connective tissues and plays an important role in vision. While X-ray scatteringtechniques are able to determine bulk structure of cornea, second harmonic generation microscopy can reveal depthdependentdetails of the corneal stroma comprised of a layered network of fibrillar collagen. In this work, we used FastFourier Transform second harmonic generation microscopy as a tool to determine the directionality of corneal stroma asa function of depth. Our results also display the position dependent difference of corneal stroma architecture.