Toxin-Antitoxin Stabilised Biosensors in the Commensal Escherichia coli Nissle 1917 for the Detection of Dysbiotic Changes in the Intestinal Microbiota

摘要

The rapid progress observed within the field of synthetic biology over the last decade has facilitated the design and creation of more clinically relevant and complex genetic circuits. The human microbiota consists of the various microorganisms associated with the human body and it is increasingly being implicated as a regulator of health and disease. The commensal nature of the intestinal microbiota and its constituents provide a number of well-tolerated microorganisms such as Escherichia coli NISSLE 1917 (EcN) that with synthetic biology could potentially exploit the intestines as an entry point and be used for monitoring or engineering the host microbiota.