Application of Reverse Sample Genome Probing to the Identification of Sulfate-Reducing Bacteria

摘要

Reverse sample genome probing (RSGP) has been used for the analysis of sulfate-reducing bacteria (SRB) in samples obtained from heavy oil operations in the Wain-wright/Wildmere fields in Western Canada. These reservoirs are shallow (600 m), roughly 25 deg C, and host a 6 percent brine. Master filters containing up to 35 independently hybridizing SRB standards were used for analysis of DNA prepared from the samples. The standards represented the diversity of SRB that is now recognized, for example, SRB using lactate, ethanol, benzoate, propionate, decanoate, or acetate as their carbon and energy source.