Applying DNA marker to detect R-genes of nematode-resistance among potato varieties and interspecies hybrids

摘要

The varieties and interspecies hybrids of potato selected on the basis of Ural Scientific Research Veterinary Institute Federal State Budgetary Scientific Institution "Ural Federal Agrarian Scientific Research Centre, Ural Branch of the Russian Academy of Science" (Ural national research institute of agriculture - the branch of USRVI FSBSI "UFASRC, UB of the RAS") have been studied with the application of three DNA markers. Based on the results of the study the data on the genes resistant (collection) to the golden cyst nematode was obtained on the basis of two markers and a pale cyst nematode on the basis of one marker with the purpose to select the specified parameters. The use of DNA markers with the high accuracy enabled to identify interspecies hybrids with R-genes. The group of potato variations with resistance to the nematodes under research according to three markers has been distinguished. The high saturation of the collection with the genes from S. andigenum to marker TG-689 from H1 gene to golden potato nematode which arc found among 82% of the collection specimens was stated. The potato variations classified as slightly affected (up to 5 cysts per a root) according to the Russian scale are characterized by containing the genes of resistance to golden potato nematode in the genotype, which enables to equate these variations with the resistant ones.