A Novel rRNA Depletion Method to Enable Whole Transcriptome Analysis of Single Cells with RNA-Seq

摘要

RNA-Seq analyses of transcripts from large numbers of cells often mask biologically relevant differences that occur in individual cells. Understanding RNA expression in a single cell allows for greater understanding of developmental processes, disease progression, and biological pathways. We will describe a novel approach for generating stranded total RNA-Seq libraries from single human cells or from input amounts of as little as 10 pg of purified RNA.