首页> 外文会议>IEEE Symposium on Business, Engineering and Industrial Applications >Comparative study between BD Falcon#x00AE; and Corning#x00AE; cell culture flask on viability and growth of breast, colon, liver and lung cancer cell lines
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Comparative study between BD Falcon#x00AE; and Corning#x00AE; cell culture flask on viability and growth of breast, colon, liver and lung cancer cell lines

机译:BD Falcon&#X00AE之间的比较研究; 和康宁® 细胞培养瓶对乳腺,结肠,肝脏和肺癌细胞系的生存和生长

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Cancer is one of the greatest threats in the world affecting millions of people worldwide. In order to treat the disease, several methods have been designed including the use of cancer cell line research model in developing the most effective drugs. However, external environment of cancer cell lines e.g. substance of flask's surface may affect the investigation. Subsequently, the results are probably not valid for further study i.e. in giving positive false results. Therefore, in this study, human colonic (HCT116), human breast (MCF7), human lung (A549) and human liver (HepG2) cancer cell lines were cultured in two most common flasks i.e. Corning Costar ® and BD FalconTM. Viability and growth of the cells was observed using trypan blue staining and an inverted microscope. There is no significant difference in viability and growth of cancer cell lines which were cultured in different flasks. It suggests that cancer cell lines are robust and can grow in both cell culture flasks successfully.
机译:癌症是世界上有数百万人的世界上最大的威胁之一。为了治疗这种疾病,设计了几种方法,包括使用癌细胞系研究模型在开发最有效的药物中。然而,癌细胞系的外部环境例如。烧瓶表面的物质可能会影响调查。随后,结果可能对进一步研究不适用于进一步研究。在提供积极的假结果时。因此,在本研究中,在最常见的烧瓶中培养人肠道(HCT116),人乳房(MCF7),人肺(A549)和人肝(HepG2)癌细胞系。和BD Falcon tm 。使用台盼蓝染色和倒置显微镜观察细胞的活力和生长。在不同烧瓶中培养的癌细胞系的活力和生长没有显着差异。它表明癌细胞系是稳健的,并且可以成功地在两种细胞培养瓶中生长。

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