Anti-proliferative effect of Tinaspora crispa (L.) Hook. F. Thompson and Gelam (Melaleuca sp.) honey on several cancer cell lines

摘要

Tinaspora crispa and Gelam (Melaleuca sp.) honey were identified as potential anti-proliferative agents due to presence of high antioxidant activity. In this research, human breast adenocarcinoma (MCF-7), human colorectal carcinoma (HCT116), human hepatocellular carcinoma (HepG2) and adenocarcinoma human alveolar basal epithelial (A549) cell lines were treated with both extracts and further diagnosed the viability using 3- (4,5 - dimethylthiazol-2-y) - 5 - (3-carboxymetaxyphenyl) 2- (4- sulphophenyl) - 2H - tetrazolium (MTS) assay. T.crispa and Gelam honey have showed anti-proliferative effects to selected cancer cell lines. The maximal effect of T. crispa and Gelam honey were demonstrated on HepG2 (IC50 = 9.13 µg/ml) and HCT116 (IC50 = 3.98 %, v/v) accordingly. In addition, we revealed that Gelam honey exhibited anti-proliferative effect on MCF-7. While the finest preparation of T.crispa crude extract revealed a potential result compared to previous studies. Total phenolic content may play the major role in demonstrating anti-proliferative effect, which was measured parallel as the main source of antioxidant activity.