Growth Kinetic of Bacillus subtilis for Pyrene biodegradation

摘要

Biodegradation of pyrene compound was catalyzed by multi-component enzymes,and the initial dioxygenase gene was used as key Bacillus subtilis C19 enzyme for aromatic ring structure degradation.The bacterial cell growth rates were also analyzed;;it was fitted using Monod,Mason-Mallis,Haldane,Andrews,and Aiba kinetics models.The un-competitive inhibition mechanism of high substrate concentration proposed by Andrews model could be best fitted to experimental bacterial growth kinetic data.Further,the maximum specific growth rate(Umax)has been found to be 0.0048 h"1,the half velocity constant(Ks)was 0.0079 gL"1,and the inhibition growth rate coefficient(Ki)was 0.2619 gL"1.