Improvement silage nutritive and hygienic value using viable lactic acid bacteria inoculant

摘要

This paper investigates the effect of adding two different inoculant blends L.buchneri, L.plantarum, P.acidilactici (II) and L.buchneri, L.plantarum (12) to medium wilted perennial ryegrass [Lolium perenne L.) on fermentation variables, microbial composition and aerobic stability of the resulting silage according to the DLG (Deutsche Landwirtschafts-Gesellshaft e.V. / internationally acknowledged German Agricultural Society) (90 d after ensiling for Objective land 49 d after ensiling for Objective 2)using an in vitro set-up with mini silos. Additives II and 12 increased (P < 0.05) the dry matter (DM) concentration, crude protein content and decreased (P < 0.05) in silo DM losses to a similar extent compared to the untreated control silage in both Objective 1 and Objective 2. Treatments II and 12 significantly increased fermentation rate of silages, resulting in a significantly better pH drop 3 d, 90 and 49 d after ensiling compared to the control silages, and produced significantly more lactate. Ammonia-N, alcohols, and butyric acid concentrations in II and 12 silages were significantly lower compared to control silage. Inoculant II produced more {P < 0.05) lactic acid in Objective 1 and Objective 2, and less acetic acid in Objective 1. Treatments significandy reduced the amount of lactate reducing yeasts and of moulds compared to control silage. Inoculant 12 was more effective for improving silage aerobic stability than II.