Comparative Properties of Hyaluronic Acid Hydrogel Cross-linked with 1, 4-Butanediol Diglycidyl Ether Assayed Using a Marine Hyaluronidase

摘要

Hyaluronic acid (HA) hydrogels for injection were fabricated using 1, 4-butanediol diglycidyl ether (BDDE) as a cross-linker. We used hyaluronidase fermented by the marine bacteria Arthrobacter globiformis A152, to comprehensively investigate the effects of BDDE concentration on swelling degree, modification degree, morphology, HA content, anti-enzyme ability, and residual free cross-linker. The results showed that within a certain range, the measured modification degrees of the HA hydrogels and HA contents changed linearly with increased concentration of the cross-linker. However, the change in anti-enzyme ability was not linear, potentially reflecting the influence of the spatial network structure.