Body has a great immune system among others of lymphocytes population. The biggest parts are T (timus) cell, B (bone) cell and Natural Killer cell (NK). T CD8~+ cell is destroying cell infected by virus and tumor cell, while NK cell is natural killer cell in mechanism of inflammatory responses. Cupping therapy is a treatment method with the principle of trauma to the skin in the form of cupping and wounding. The general purpose of this research is to prove the effect of cupping on the enhancement of T CD8~+ and NK cells as inflammation responses. The research design is quasi experimental by using nonrandom pretest - posttest without control group design that is done towards human being as the research subject. The treatments of cupping therapy are done in Jember for two weeks on the twenty people research subjects. All of the research subjects are taken their venous blood as much as 2ml in the median cubiti vein area then are done cupping therapy in seven points. Cupping areas are Al-Akhda'ain 2 points, Al-Kahil 1 point, Al-Katifain 2 points and Ala Warik 2 points. The next blood samplings are in the fiveteenth day. Blood checking uses flow cytometry is done in Laboratory of Medical Faculty in Brawijaya University of Malang. After collecting the data, data analyses are done computerized by using ANOVA test because it is homogeny and the normality test is fulfilled. The measurement results obtained T CD8+ cell mean values in pretreatment was 34.56, in post treatment mean value was 63.26, standard deviation was 7.41 with p value was 0.00 smaller than 0.05. In pretreatment, NK cell mean value was 16.25 and in post treatment mean value was 35.50, standard deviation was 5.17, the test was using ANOVA obtained p value was 0.00 < 0.05. There was significance difference in value of T CD8+ and NK cells in pre post treatments. So, cupping therapy is able to stimulate the inflammation response by producing T CD8+ and NK cells.
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