A First Epidemiological Survey of Skin Fungus in Free Range Pelophylax Nigromaculatus in Hunan Province, China

摘要

Epidemiological investigation of fungus in amphibian was conducted from Hunan province of China to isolate fungus from skin of amphibians. Specimens from the skin of local Pelophylax Nigromaculatus were collected and analyzed using molecular biology techniques. The sequences were analyzed using phylogenetic tree. From the Pelophylax Nigromaculatus specimen collected a total of 58 fungal strains representing 10 fungi species from 8 genera were identified. The detection rate of Penicillium spp. was highest (11%), followed by Yarrowia spp. (1.5%) and detection rate of other genera varied from 0.25% ～ 0.75%. Therefore, long-termmonitoring should be implemented to realize ecological risk for Hunan province and vital to establish a necessary management measures for monitoring and prevention of pathogenic fungus which have great significance in protecting ecological risk in China as well as eastern Asian regions.