Study on Extraction Technology and Functional Activity of Sika Deer Velvet(Residue) Collagen

摘要

Objective:Extraction method and functional activity of collagen that is extracted from residue that is insoluble in any medium polar solvent by ultrasonic extraction and supercritical CO_2 extraction are studied. Finally the optimal enzymolysis conditionsare obtained. Methods: Quantitative content of collagen in deer antler (residue) by quantitate hydroxyproline. Sika deer antler collagen is prepared by enzymolysis of papain. According to single factor test and the orthogonal design, the optimal enzymatic conditions aredetermined by the extraction rate of collagen as evaluation indicator. And functional activity are tested which include antioxidant capacity, hydroxyl free radical scavenging ability, DPPH scavenging ability of the test and keratinocyte cell test. Results: The content of collagen in the deer antler (residue) is 4.5795%, the best extraction process is 2% optimum enzyme dosage, PH 6.0, 1:20 solid-liquid ratio, enzymolysis time 66h at 10°C. The extraction rate of collagen of the deer antler is 54.59%. The protein is collagen by UV spectroscopy. Functional tests are showed a good functionality and growth and reproduction capacity.