Tissue Culture of Regeneration Induced by Leaves of 'Hongyang'

摘要

The test combined the concentration in 0.5mg.L~(-1), 1.0mg.L~(-1), 1.5mgL~(-1) of 1-naphthaleneacetic acid (NAA) and a concentration of 2.0mg.L~(-1), 2.5mg.L~(-1), 3.0mgL~(-1) of 6-benzylaminopurine (6-BA), from which we got a comprehensive evaluation of shoots survival ratio, growing point proliferated rate and shoots growth status together. From all the treatment we selected MS + 1.0mg.L~(-1) NAA + 3.0mg.L~(-1) 6-BA for the optimum combination, for its live ratio of budding leaves on 92.59%, growing point proliferation rate on 629.60%. In this experiment, each treatment adventitious buds were over 300.00%, that all the treatments are able to use as the media formulations for proliferation of adventitious buds.