A PCR-DGGE approach to evaluate the influence of environment materials for civil engineering-different carbon sources on a catching bed biofilm reactor in start-up phase

摘要

In this study, the effect of environment materials for civil engineering was investigated through two sets of parallel reactors with sodium acetate and starch as carbon source separately. The reactors were started up by two stages, including biofilm formation stage and biofilm domestication stage. In biofilm domestication stage, the average removal efficiencies of COD were 88.84% and 88.78% respectively. (NH_4)~+-N removal efficiencies were reached at 99.28% and 91.90%. Total nitrogen (TN) removal efficiencies were 45.25% and 45.45%. By PCR-DGGE, it was found that there were high similarities between biofilm and suspension in the reactors. Different types of carbon sources enriched different types of microorganisms. The study can provide references for studying simultaneous nitrification and denitrification (SND) and optimizing carbon sources.