New microsatellite DNA primers for Chimonanthus grammatus peculiarly distributed in southern China

摘要

Chimonanthus grammatus M. C. Liu is an endangered and national-protected medicinal plant species with highly restricted geographical distribution and disjunctly locates in the border area of Anyuan and Huichang county of Jiangxi province, China. In this study, we developed ten microsatellite loci for this species for the first time. Nine of them was screened by combining biotin capture method to enrich (CA)15/(GA)15 microsatellites, and one primer is designed from one EST sequence (DW222670) of Chimonanthus praecox. Ten microsatellite primers were found to be polymorphic through screening 30 individuals. Na was from two to eighteen. Ho and He ranged from 0.233 to 0.933 and 0.326 to 0.898, respectly. Polymorphic information content (PIC) was from 0.316 to 0.890. Ten microsatellite primers will be useful to investigate the genetic structure of Chimonanthus grammatus M. C. Liu and to explain its morphological differentiation from another species in Chimonanthus.