Effect of Pre-treatment Palm Oil Mill Effluent POME, on Biohydrogen Production by Local Isolate Clostridium butyricum

摘要

Palm oil mill effluent (POME) contains approximately 6% fiber. The effectiveness of pre-treatment on POME can serve a very good feedstock for hydrogen production in fermentation process. In this research, the effectiveness of pre-treatment methods on POME treated using acid and base were analysed based total carbohydrate and reducing sugar content. By using 1M NaOH with heat treatment, 26.12% carbon source converted to reducing sugar while by using 1M H2SO4 with heat treatment, over 32.09% carbon source converted to reducing sugar. The highest increment of total carbohydrate where from acid-heat treatment with 26.1% increment from initial concentration.