In vitro Release Characterization of Tetracycline Hydrochloride from PLGA/Tetracycline Hydrochloride Electrospun Nanofiber Mats

摘要

In order to study in vitro release behavior of hydrophilic drugs in polymer nanofibers and establish a fast characterization method, tetracycline hydrochloride-loaded poly(lacticacid-co-glycolic acid) nanofiber mats with varied tetracycline hydrochloride contents and different lacticacid to glycolic ratios in PLGA were preparied by electrospinning. Accroding to Chinese Pharmacopoeia, a basic and an improved test devices and processes were compared and their validity were commented by using an UV-visible spectrophotometer method. Results showed that the improved method can be used to estimate in vitro drug release behavior of drug-loaded mats and the results was better than the basic method. When temperature affected cumulative release ratio under controlled error, flotation method can replace the centrifugation method during the first stage of drug release testing process. Parallel experiments were carried out and results indicated that nanofibres on different part of mats had a relative stable result and repeat experimental error was kept below 4%.