Optimization of liquid fermentation for mycelial growth and extracellular polysaccharide production by Poria Cosos

摘要

This paper is an attempt to study the growth line of poria cocos through flask culture experiment, and leads to a discussion of different fermentation conditions. The optimum media for mycelial growth were determined as (g/l): glucose 60, peptone 10, KNO_3 10, KH_2PO_4 3, and MgSO_4 1; while for exopolysaccharide was (g/l), glucose 40, peptone 5, KNO_3 5, KH_2PO_4 0.5, MgSO_4 1. The optimum initial pH value of medium was 5.5, inoculation, 10% (v/v) and different liquid volume had minimal influence on the mycelial growth and exopolysaccharide production.