Analysis of Microbial Community Structure in a Novel two-sludge Denitrifying Phosphorus Removal Process Using Fluorescence in Situ Hybridization (FISH)

摘要

The microbial communities in a novel two-sludge denitrifying phosphorus removal process were investigated by using FISH targeting the 16SrRNA of polyphosphate-accumulating organisms (PAO), ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB), and DAPI chemical staining. The microbial morphology was also analyzed by SEM. It was found that PAO was effectively enriched in anoxic tank, occupying 46.7 of total bacteria. In nitrification tank, the numbers of AOB were higher than that of NOB, accounting for 46.2% of total bacteria and 28.5% of that. There was a strong difference of microbial morphologies between anoxic tank and nitrification tank. A variety of rod morphology microorganisms was found in anoxic tank, while nitrification sludge consisted of clusters of microorganisms. These results suggesting that the novel two-sludge can provide a benefit for the cultivation of PAO or nitrifiers compared with the single sludge.