Rapid Preparation of Pure Antibodies Against Classical Swine Fever Virus from Pig Serum by Immunoaffinity Chromatography

摘要

After Sepharose-4B polymerbeads were activated by using epichlorohydrin, purified swine fever virus as a ligand were binded with them to prepare an immobilized affinity chromatography column, which was used to prepare antibody from high immune pig serum. Equilibrated with pH 7.4, 0.02 mol/L PBS and eluated with pH 3.4, 0.2 mol/L NaAc-HAc buffer containing 0.5 mol/L NaCl, the purified protein obtained from this columne was demonstrated to have normal activity to combine with classical swine fever virus by SDS-PAGE and ELISA. The extraction efficiency of the antibody was 2.45% of total proteins. This study offers a novel, rapid and effective method for preparation of pure antibodies against classical swine fever virus from high immune pig serum.