Fabrication of Angiogenic Gene-modified Myoblast Cell Sheets using Magnetic Tissue Engineering Techniques

摘要

We present the fabrication of angiogenic gene-modified tissue constructs by applying two magnetic biomanipulation techniques using magnetite cationic liposomes (MCLs) to gene transfer and tissue fabrication processes. For gene delivery, the vascular endothelial growth factor (VEGF) gene was transduced to mouse myoblast C2C12 cells using a magnetofection technique in which retroviral vector/MCL complexes and magnetic force were used for gene transfer. This method enhanced the transduction efficiency by 8.4-fold compared with the infection without MCLs, while the conventional method using polybrene slightly improved the transduction efficiency. During the tissue fabrication process, multilayered cell sheets were fabricated by accumulating MCL-labeled C2C12 cells under a magnetic field. When the VEGF gene-engineered cell sheets were transplanted subcutaneously into nude mice, vascularization was promoted within the graft and thick tissue formation was observed. These results indicate that magnetic biomanipulation techniques can be effective tools for tissue engineering.