Interaction of Sulpiride and Serum Albumin: Modeling from Spectrofluorimetric Data

摘要

We have applied the fluorescence quenching modeling to study the process of interaction of sulpiride with human serum albumin (HSA) and bovine (BSA). Albumin is more abundant protein in blood and it emits fluorescence when excited by 260-295 nm. Sulpiride is an atypical antipsychotic used in the treatment of many psychiatric disorders. As sulpiride is fluorescent, we developed a mathematical model to analyzing the interaction of two fluorescent substances. This model was able to separate the albumin fluorescence from the quencher fluorescence. Results have shown that sulpiride quenches the fluorescence of both albumins by a static process, due to the complex formation drug-albumin. The association constants calculated for sulpiride-HSA was 2.20 (± 0.08) × 10~4 M~(-1) at 37° C, and 5.46 (± 0.20) × 10~4M~(-1), 25 ° C, and the primary binding site to sulpiride in the albumin is located closer to the subdomain IB.