FLEXIBLE AND ABSORBENT COTTON-ALGINATE WOUND DRESSING

摘要

The lowering of high protease activity in the chronic wound is an important therapeutic route to accelerated healing with active chronic wound dressings. A cotton-based chronic wound dressing has been developed based on molecular design features thatinclude the binding affinity of destructive proteases by charge and active site interaction. The active wound dressing's design is tailored for selective uptake of human neutrophil elastase (HNE). Phosphorylated cotton gauze was designed and prepared based on a molecular mechanism for serine protease sequestration and a one-step aqueous textile finishing process. Cotton gauze containing 3-4% phosphorylated cellulose was found to lower elastase activity compared with untreated gauze. The elastase-lowering activity of the phosphorylated cotton in-situ was effective with wound dressing incubations for one, six, and twenty-four hours. The phosphorylated gauze also lowered other serine and matrix metallo proteases. The in-situ monitoring of elastase activity in the presence of phosphorylated cotton gauze was determined by assessing the kinetics of substrate hydrolysis in a two-phase system. The in-situ determination of an elastase-lowering effect with the phosphorylated gauze demonstrates that the enzyme is irreversibly bound by the phosphorylated cotton wound-dressing fiber. Development of the wound dressing preparation on a continuous process scale suitable for textile mill production of the gauze required adapting the laboratory process for preparationof the phosphorylated cotton gauze in a padder and oven configuration. The prevention of pressure ulcers with modified cotton bed sheets is also considered here based on treatment of cotton sheets to confer properties of water transport, high adsorptioncapacity, resilience, softness, crease resistance, and antibacterial activity. A fabric hand laboratory analysis (the Kawabata Evaluation System) was used to determine compressive resilience and surface properties related to friction and surface roughness.