Interaction of Antiprotozooal Drugs with Biomolecules. An ESR and UV-vis Spectroelectrochemical study

摘要

Cyclic voltammetric study in aprotic medium of antiprotozooal nitro compounds, Nifurtimox, Benznidazol and Niclosamide in the presence of glutathione and triolein, compounds that mimic the cellular constituents of the living being, were carried out in order to investigate the interactions of these compounds with the electrogenerated intermediaries in the reduction of the nitro compounds such as radical anion, radical dianion and hydroxylamine, whose could be obtained through electrochemical methods. Glutathione caused an important shift potential of the dianion radical (peak IIc) of nifurtimox and benznidazol and a minimum interaction with the radical anions (peak Ic). However, a significant change in the voltammetric behavior of nifurtimox was observed for both peaks Ic and IIc with triolein. Niclosamide presented more complicated responses. ESR and UV-vis experiments with nifurtimox showed that the nitro radical anion is interacting with triolein, even at small concentrations.