Radiation-induced Triticumaestivum-Leymus racemosus translocations and their molecular cytogenetic analysis

摘要

Fourteen alien translocations between common wheat and Leymus racemosus were developed via using ~(60)Co-y rays irradiate premeiotic adult plants or pollen of the scab-resistant addition lines with the added chromosome Lr.2, Lr.7, and Lr.14 of L racemosus. Giemsa C-banding and fluorescent in situ hybridization analysis identified TOl and T14 as containing largely wheat, partly-ahen translocated chromosomes; T02, T03, T04, and T13 have largely alien, partly-wheat translocated chromosomes; T05, T06, T07, T08, T09, T10, and T12 contain translocated chromosomes with breakpoints at or near centromere; and Til has both a mostly alien and a mostly wheat translocated chromosome.