A Step Closer to the Market for Poly-hydroxy-alkanoates(PHAs)through their Bacterial Production in Continuous Bioreactors in Series:a Model Investigation of Novel Operating Strategies

摘要

The current work explores the dynamic optimization of a sequence of two continuous stirred-tank bioreactors for the production of poly-3-hydroxybutyrate(PHB)in Azohydromonas lata cultures using a credible and sophisticated mathematical model.The cumbersome problem of operating a microbial fermentation process continuously in a consistent,systematic and controllable manner is uncovered at its full dimension and addressed,pursuing in parallel the efficient substrate utilization and the optimization of the fermentation stage.Advanced optimization algorithms and well-posed optimization strategies with different subsets of control variables are implemented A notable improvement on the performance of the continuous optimal operating bioreactors is demonstrated via the maximization of key process factors,such as productivity,biopolymer content,final product concentration etc.,under three different strategies