Lab-on-a-Chip Ochratoxin A Detection Using Competitive ELISA in Microfluidics with Integrated Photodiode Signal Acquisition

摘要

Ochratoxin A (OTA), a mycotoxin which occurs naturally in a variety of crops (e.g. cereals, grapes), can contaminate feedstock and beverages derived from these crops (e.g. wine and beer) [1]. OTA detection is critical for food safety since exposure through consumption is related to potential health risks, namely cancer [2]. Food and beverages commercialized in Europe must follow the European Commission regulations for the amount of OTA allowed. In this work, OTA detection was developed and performed based on indirect competitive enzyme linked immunosorbent assays (icELISA) perfomed inside polydimethylsiloxane microchannels. The microfluidic icELISA is integrated with hydrogenated amorphous silicon photodiodes microfabricated on a glass substrate for a fast, reliable, and portable OTA detection and quantification system.