Genetic variation in an immigrant population (british pakistanis) and its comparison with the origin pakistani population by using str dna profiling

摘要

Allelic frequencies and forensic parameters for autosomal Short Tandem Repeats (STR), as well asgenetic structure were determined in two populations. A total of 209 samples, 96 from British Pakistanis fromUK cities and 113 from Pakistani cities were PCR-typed for 15 autosomal STR by using AmpF?STR? IdentifilerApplied Biosystem Kit. Powerstat?V12 software was used for data interpretation. GENPOP 4.0.10 linkagedisequilibrium and Arlequin version 3.5 P values for HWE equation were calculated. The genotype of each locuswas in Hardy-Weinberg equilibrium for 15 STRs in both populations after Bonferoni correction except:D8S1179 and D18S51 for the Pakistani population, and D19S433 and D5S818 for the British Pakistanipopulation. In the Pakistani population, the power of discrimination (PD) values for the tested loci ranged from0.862 at the CSF1PO locus to 0.978 at the D19S433 locus. In the British Pakistani population, the PD values forthe tested loci ranged from 0.866 at the TPOX locus to 0.974 at the FGA locus. The combined power ofdiscrimination and exclusion for the British Pakistani and Pakistani populations were all ≥0.99999. A slightlyincreased rate of homozygosity in the British Pakistanis may suggest more consanguinity and susceptibility toautosomal recessive disorders compared to the Pakistani population. The FST between these two populations was0.02288.