A NOVEL APPROACH IN THE USE OF FOOD GRADE YEAST AS A NATIVE STANDARD FOR THE DEVELOPMENT OF PRECISION AND BIAS FOR A RAPID ATP BIOLUMINESCENCE ASSAY METHOD

摘要

When determining the precision and bias associated with an analytical test method many factors must be identified and considered in order to reduce or eliminate sources of systematic error in order to have an effective and robust test protocol. This includes sample collection, sample preparation, harvesting the analyte of interest, detecting the analyte of interest, and accounting for sample matrix effects to name a few. In some instances, circumstances may prevail where an analytical test protocol must be validated by inference or by surrogate technique because the analyte of interest is not available commercially or is not easily utilized in its "native" form as found in the field. Such was the case with ASTM D4012 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water. In this method the author admittedly relied on the analytical grade of the target analyte ATP because live test standards were not available in a workable and stable "native" form. Now that ATP Bioluminescence Assay has gained wider acceptance for use in the fuel industry, this new microbial analytical technique must be challenged using standards that are as close to the active "native" form in the proper sample matrix or matrices. This paper describes a novel approach to utilize a commercially available food grade yeast to research and develop a stable ATP standard that could be utilized in its "native" form in the various sample matrices found in the field so as to challenge the validity and robustness of a ATP Rapid Bioluminescence Assay, ASTM D7463 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water.