CHARACTERIZATION AND COMPARISON OF O-GLYCOSYLATION BETWEEN THE INNOVATOR AND A BIOSIMILAR OF ETANERCEPT

摘要

Developed a sample preparation workflow to study Core1 O-glycoproteins. Identified 5 tryptic peptides containing O-linked glycans (see table1). Assigned 12 glycosylation sites on 9 glycopeptides from Etanercept (see table2). Highlighted differences in glycosylation patterns between the innovator and a biosimilar. Synapt G2-S MS system with ETD can be routinely used for O-glycopeptides sites analysis of protein therapeutics.