Glycoproteomic identification and characterization of potential glycoprotein biomarkers in ovarian cancer proximal fluids

摘要

1. Through the use of glycoproteomic techniques we have identified the sialylated subset of the ovarian cancer secreted or shed glycoproteome, and identified a candidate list of glycoproteins manifesting differential sialylation patterns between malignant and non-malignant samples. 2. Kallikrein 6 from derived from ovarian cancer cells exhibits differentially expressed glycosylation patterns. 3. The glycosylation patterns of ovarian cancer-related kallikrein 6 are highly heterogeneous with a propensity for increased branching and sialic acid content. 4. The changes in kallikrein 6 in serum and other biological fluids can be monitored by an HPLC anion-exchange ELISA-coupled methodology developed as a part of this study.