Screening analysis for drugs of abuse by LC-MS/MS enables fast polarity switching MRM triggered product ion scanning on the fly

摘要

1. A high speed LC/MS/MS data acquisition system was applied to drug screening in forensic, toxicological and clinical analysis. 2. To achieve a highly specific and sensitive detection method in screening and quantitation, an MRM triggered product ion scanning method using a polarity switching speed of 15msec and a scan speed of 15,000u/sec was applied to 111 components including illicit drugs, psychotropics, hypnotics, pesticides and other substances. 3. As the MRM acquisition time was very fast, this enabled product ion spectra to be generated in both positive and negative ionization mode which could be matched against a user library of compounds as an automated aid to screening and compound identification.