CONCURRENT QUANTIFICATION OF SYNAPTOTAGMIN-1 AND SNAP-25 IN CLINICALLY RELEVANT VOLUMES OF CSF BY IMMUNOAFFINITY LC-MS/MS

摘要

Reduction of synaptic activity is an early event in several neurodegenerative diseases. In two earlier studies we have used affinity purification combined with PRM-MS to demonstrate increased CSF levels of the synaptic proteins synaptotagmin-1 and SNAP-25 in patients with Alzheimer's disease compared with controls(1, 2). In this study the strategy was further developed to concurrently measure levels of both proteins in individual patient CSF samples.